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In biology and medicine, in addition to the more traditional uses described above, serial dilution can also be used to reduce the concentration of microscopic organisms or cells in a sample.
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Typically, the dilution factor is constant at each step, resulting in a logarithmic geometric concentration curve. Serial dilution is the gradual dilution of a substance in solution. A predetermined amount of the original sample is inoculated into several tubes. What is the serial dilution method? What is a serial dilution test?Ī serial dilution test measures the concentration of a target microbe in a food or water sample. If a 1/100 diluted sample is added to a 1/10 diluted sample, the final dilution will be: (1/100) x (1/10) = 1/1000. How are dilution series calculated in microbiology? Serial dilutions are the culmination of a series of dilution tubes used to make smaller dilutions. How do you calculate serial dilutions in microbiology The diluted sample is then used as a stock solution for further dilution. How do you perform a serial dilution?įor serial dilution, a small amount of the well-mixed solution is transferred to a new container and additional water or other solvent is added to dilute the stock solution. Calculate the amount of solution that goes from one dilution to another. Determine how much solution you need for each dilution. They hope this helps you along with the serial dilution definition above - determine the dilution amount, dilution factor (or range), and stock concentration. The dilution factor or dilution factor is the initial volume divided by the final volume. For serial dilutions, multiply the dilution factors for each step. Serial dilution is any dilution where the concentration is reduced by the same factor in each successive phase. Serial dilution is a series of serial dilutions with a constant dilution factor at each step. How do you calculate the serial dilution? Dilution calculations can be performed using the formula M 1 V 1 = M 2 V 2.